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KMID : 1101420180500040501
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Korean Journal of Clinical Laboratory Science
2018 Volume.50 No. 4 p.501 ~ p.510
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Alleviating Effects of Euphorbiae humifusae L. Extract on the Neurotoxicity Induced by Lead
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Lee Sang-Hee
Seo Young-Mi
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Abstract
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This study examined the neurotoxicity induced by lead acetate (LA) on cultured C6 glioma cells and the protective effects of Euphorbiae humifusae L. (EH) extract against LA-induced cytotoxicity. In this study, LA exhibited neurotoxicity in a dose-dependent manner compared to the control, and was determined to be highly-toxic according to the toxic criteria. The XTT50 value of LA was calculated at a concentration of 38.6 ?M after C6 glioma cells were incubated for 72 hours in the media containing 30∼50 ?M of LA, respectively. In addition, LA-induced neurotoxicity was suggested to correlate with the level of oxidative stress because vitamin E, an antioxidant, increased the cell viability damaged by LA-induced cytotoxicity. The EH extract effectively prevented cell injury from LA-induced cytotoxicity via its antioxidative effects, such as inhibitory ability of lipid peroxidation, superoxide dismutase-like activity and 1,1-diphenyl-2-picrylhydrazyl-radical scavenging activity. These antioxidative effects may result in components, such as polyphenol or flavonoids including gallic acid or quercetin. In conclusion, natural resources, such as EH extracts, may be a useful putative agent for the treatment of diseases associated with oxidative stress, such as lead neurotoxicity.
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KEYWORD
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Antioxidative effect, Neurotoxicity, Oxidative stress
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